Anticorps Polyclonal de lapin anti-RTN3

RTN3 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12055-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12055-2-AP (same clone as 12055-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12055-2-AP (same clone as 12055-2-PBS)

  IP result of anti-RTN3 (IP:12055-2-AP, 4ug; Detection:12055-2-AP 1:400) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

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Informations sur le produit

12055-2-PBS cible RTN3 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTN3 Protéine recombinante Ag2685
• Nom complet: reticulon 3
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 24 kDa, 100-113 kDa
• Numéro d’acquisition GenBank: BC011394
• Symbole du gène: RTN3
• Identification du gène (NCBI): 10313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

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