Anticorps Monoclonal anti-RTN3

RTN3 Monoclonal Antibody for WB, IP, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E3H11

N° de cat : 68215-1-PBS

Synonymes

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

---

Galerie de données de validation

• 

  WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50025-1

  Sandwich ELISA standard curve ofMP50025-1, RTN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68215-2-PBS. Detection antibody: HRP-conjugated 68215-1-PBS. Standard: Ag13766. Range: 0.625-20 ng/mL.

• 

  IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

  IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68215-1-PBS cible RTN3 dans les applications de WB, IP, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RTN3 Protéine recombinante Ag13766
• Nom complet: reticulon 3
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC011394
• Symbole du gène: RTN3
• Identification du gène (NCBI): 10313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

{{ptg:RelatedPrimaryAntibodies}}