Anticorps Recombinant de lapin anti-RUFY1

RUFY1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250159A3

N° de cat : 85839-4-PBS

Synonymes

FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, RABIP4, RUFY 1

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Galerie de données de validation

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  WB analysis using 85839-4-RR (same clone as 85839-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85839-4-RR (RUFY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 85839-4-RR (same clone as 85839-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RUFY1 antibody (85839-4-RR, Clone: 250159A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85839-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85839-4-RR against Human RUFY1 were performed. The affinity constant is 0.444 nM.

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Informations sur le produit

85839-4-PBS cible RUFY1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RUFY1 Protéine recombinante Ag4324
• Nom complet: RUN and FYVE domain containing 1
• Masse moléculaire calculée: 708 aa, 80 kDa
• Poids moléculaire observé: 80 kDa, 69 kDa
• Numéro d’acquisition GenBank: BC032571
• Symbole du gène: RUFY1
• Identification du gène (NCBI): 80230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

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