Anticorps Recombinant de lapin anti-RUFY1

RUFY1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250159A3

N° de cat : 85839-4-RR

Synonymes

FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, RABIP4, RUFY 1

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Galerie de données de validation

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  WB analysis using 85839-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85839-4-RR (RUFY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 85839-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RUFY1 antibody (85839-4-RR, Clone: 250159A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85839-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85839-4-RR against Human RUFY1 were performed. The affinity constant is 0.444 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85839-4-RR cible RUFY1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RUFY1 Protéine recombinante Ag4324
• Nom complet: RUN and FYVE domain containing 1
• Masse moléculaire calculée: 708 aa, 80 kDa
• Poids moléculaire observé: 80 kDa, 69 kDa
• Numéro d’acquisition GenBank: BC032571
• Symbole du gène: RUFY1
• Identification du gène (NCBI): 80230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

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