Anticorps Polyclonal de lapin anti-RUFY2

RUFY2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 19180-1-AP

Synonymes

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

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Galerie de données de validation

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  WB analysis of mouse lung using 19180-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19180-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19180-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules SH-SY5Y, cellules U-251, tissu ovarien de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19180-1-AP cible RUFY2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUFY2 Protéine recombinante Ag5938
• Nom complet: RUN and FYVE domain containing 2
• Masse moléculaire calculée: 403 aa, 46 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC041092
• Symbole du gène: RUFY2
• Identification du gène (NCBI): 55680
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

sarah (Verified Customer) (01-02-2025)

works well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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