Anticorps Recombinant de lapin anti-RUFY3

RUFY3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240519E7

N° de cat : 83601-2-PBS

Synonymes

240519E7, KIAA0871, Protein RUFY3, Rap2 interacting protein x, Rap2-interacting protein x

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Galerie de données de validation

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  WB analysis using 83601-2-RR (same clone as 83601-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83601-2-RR (same clone as 83601-2-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00602-2

  Cytometric bead array standard curve of MP00602-2, RUFY3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83601-1-PBS. Detection antibody: 83601-2-PBS. Standard: Ag35558. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83601-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83601-2-PBS cible RUFY3 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RUFY3 Protéine recombinante Ag35558
• Nom complet: RUN and FYVE domain containing 3
• Masse moléculaire calculée: 53 kDa
• Numéro d’acquisition GenBank: BC051716
• Symbole du gène: RUFY3
• Identification du gène (NCBI): 22902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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