Anticorps Monoclonal anti-RUNX1T1

RUNX1T1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G2F7

N° de cat : 67086-1-PBS

Synonymes

Eight twenty one protein, Cyclin-D-related protein, CDR, CBFA2T1, AML1T1

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Galerie de données de validation

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  WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

  WB result of RUNX1T1 antibody (67086-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis using 67086-1-Ig (same clone as 67086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67086-1-Ig (same clone as 67086-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67086-1-Ig (same clone as 67086-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67086-1-PBS cible RUNX1T1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RUNX1T1 Protéine recombinante Ag7893
• Nom complet: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC005850
• Symbole du gène: RUNX1T1
• Identification du gène (NCBI): 862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.

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