Anticorps Recombinant de lapin anti-RUNX2

RUNX2 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

1G5

Avis (2)

N° de cat : 82636-2-RR

Synonymes

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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Galerie de données de validation

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  WB analysis using 82636-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 82636-2-RR

  IP result of anti-RUNX2 (IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules C2C12
• Résultats positifs en IP: cellules ROS1728,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

82636-2-RR cible RUNX2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: runt-related transcription factor 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: NM_004348
• Symbole du gène: RUNX2
• Identification du gène (NCBI): 860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

Publications

Species	Application	Title
mouse	WB,IHC	Int Immunopharmacol; Aging and autophagic phenotypic changes in bone marrow mesenchymal stem cells in glucocorticoid-induced osteonecrosis; Authors - Ruomu Cao
mouse	WB	Intractable Rare Dis Res; Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells; Authors - Yongtao Zhang
human	WB	Bone; CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis; Authors - Lei Li
human	WB	Arch Oral Biol; Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis; Authors - Yu Ren
rat	WB	J Orthop Surg Res; Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis; Authors - Lifang Zhang
mouse,rat	WB,CoIP	Cell Commun Signal; Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling; Authors - Xue-Xue Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-22-2024)

I used this antibody in WB in cells that differentiate into osteoblast lineage upon stimulation. I had a strong visible band at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: C3H10T1/2

RUNX2 Antibody Western Blot validation (1/2000 dilution) in C3H10T1/2 (Cat no:82636-2-RR)

FH

Udesh (Verified Customer) (11-26-2023)

Worked well for ADSCs at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ADSCs

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