Anticorps Polyclonal de lapin anti-RYR2

RYR2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(30)

N° de cat : 19765-1-AP

Synonymes

ryanodine receptor 2, cardiac, ryanodine receptor 2 (cardiac), hRYR-2, Cardiac muscle ryanodine receptor-calcium release channel, ARVD2

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Galerie de données de validation

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  WB analysis using 19765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19765-1-AP (RYR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 19765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 19765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain, tissu de muscle squelettique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

19765-1-AP cible RYR2 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ryanodine receptor 2 (cardiac)
• Masse moléculaire calculée: 565 kDa
• Numéro d’acquisition GenBank: NM_001035
• Symbole du gène: RYR2
• Identification du gène (NCBI): 6262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP). This antibody detects a band at ~250 kDa which has been reported (PMID: 22962011).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Subcellular activation of β-adrenergic receptors using a spatially restricted antagonist; Authors - Federica Liccardo
mouse	WB	Sci China Life Sci; LncRNA CCRR maintains Ca2+ homeostasis against myocardial infarction through the FTO-SERCA2a pathway; Authors - Hua Yang
mouse	WB	Mol Metab; Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor; Authors - Kelsey M Pinckard
mouse	WB	Cell Mol Life Sci; Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling; Authors - Zhi-Xing Wei
human,mouse	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen ; KD Validated
rat	WB	Part Fibre Toxicol; Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes.; Authors - Sujin Ju

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