Anticorps Polyclonal de lapin anti-RYR2

RYR2 Polyclonal Antibody for IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27587-1-PBS

Synonymes

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Galerie de données de validation

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  IHC staining of human heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 27587-1-AP (same clone as 27587-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human RYR2 (27587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27587-1-PBS cible RYR2 dans les applications de IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RYR2 Protéine recombinante Ag26296
• Nom complet: ryanodine receptor 2 (cardiac)
• Masse moléculaire calculée: 565 kDa
• Numéro d’acquisition GenBank: NM_001035
• Symbole du gène: RYR2
• Identification du gène (NCBI): 6262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP).

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