Anticorps Polyclonal de lapin anti-RanBP1

RanBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27804-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 27804-1-AP (same clone as 27804-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 27804-1-AP (same clone as 27804-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 27804-1-AP (same clone as 27804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27804-1-AP (same clone as 27804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 27804-1-AP (same clone as 27804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

27804-1-PBS cible RanBP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RanBP1 Protéine recombinante Ag25714
• Nom complet: RAN binding protein 1
• Poids moléculaire observé: 28 kDa
• Symbole du gène: RANBP1
• Identification du gène (NCBI): 5902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

{{ptg:RelatedPrimaryAntibodies}}