Anticorps Polyclonal de lapin anti-RanBP2

• Phare
• Validé par KD/KO

RanBP2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27606-1-PBS

Synonymes

358 kDa nucleoporin, ANE1, E3 SUMO protein ligase RanBP2, Nucleoporin Nup358, NUP358

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Galerie de données de validation

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  WB analysis using 27606-1-AP (same clone as 27606-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 27606-1-AP (same clone as 27606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 27606-1-AP (same clone as 27606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27606-1-PBS cible RanBP2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RanBP2 Protéine recombinante Ag26370
• Nom complet: RAN binding protein 2
• Masse moléculaire calculée: 358 kDa
• Poids moléculaire observé: 358 kDa
• Numéro d’acquisition GenBank: NM_006267
• Symbole du gène: RANBP2
• Identification du gène (NCBI): 5903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RanBP2 (Ran-binding protein 2) is also named as p270 and NUP358, and belongs to the RanBP2 E3 ligase family. RanBP2 is E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PMID:11792325, PMID:22194619). RanBP2 involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PMID:7775481). It is component of the nuclear export pathway (PMID:10078529). RanBP2 recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PMID:20386726).

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