Anticorps Polyclonal de lapin anti-RanGAP1

RanGAP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 27405-1-AP

Synonymes

SD, Ran GTPase-activating protein 1, KIAA1835

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Galerie de données de validation

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  WB analysis of SKOV-3 using 27405-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27405-1-AP (RanGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27405-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 27405-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27405-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 27405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27405-1-AP (RanGAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, cellules Neuro-2a, cellules NIH/3T3, cellules SKOV-3
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

27405-1-AP cible RanGAP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RanGAP1 Protéine recombinante Ag26128
• Nom complet: Ran GTPase activating protein 1
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa, 82 kDa
• Numéro d’acquisition GenBank: BC014044
• Symbole du gène: RANGAP1
• Identification du gène (NCBI): 5905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
mouse	WB,IF	iScience; Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain; Authors - Terry R Suk

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