Anticorps Polyclonal de lapin anti-Raptor

• Phare
• Validé par KD/KO

Raptor Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20984-1-PBS

Synonymes

KIAA1303, TOR, RPTOR, Regulatory-associated protein of mTOR, p150 target of rapamycin

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Galerie de données de validation

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  WB analysis of HepG2 cells using 20984-1-AP (same clone as 20984-1-PBS)

  WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  WB analysis using 20984-1-AP (same clone as 20984-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20984-1-AP (same clone as 20984-1-PBS)

  IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 20984-1-AP (same clone as 20984-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20984-1-PBS cible Raptor dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: raptor
• Masse moléculaire calculée: 149 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: NM_020761
• Symbole du gène: Raptor
• Identification du gène (NCBI): 57521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.

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