Anticorps Monoclonal anti-Raptor

Raptor Monoclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B8A12

N° de cat : 68286-1-PBS

Synonymes

KIAA1303, raptor, RPTOR

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Galerie de données de validation

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  WB analysis using 68286-1-Ig (same clone as 68286-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68286-1-Ig (same clone as 68286-1-PBS)

  IP result of anti-Raptor (IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68286-1-PBS cible Raptor dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Raptor Protéine recombinante Ag30131
• Nom complet: raptor
• Masse moléculaire calculée: 1335 aa, 149 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC136654
• Symbole du gène: Raptor
• Identification du gène (NCBI): 57521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

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