Anticorps Recombinant de lapin anti-Ribosomal protein L4

• Phare
• Validé par KD/KO

Ribosomal protein L4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3K6

N° de cat : 80959-1-PBS

Synonymes

RPL4, 3K6, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

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Galerie de données de validation

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  WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80959-1-RR (same clone as 80959-1-PBS)

  WB result of Ribosomal protein L4 antibody (80959-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HEK-293 cells. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

  IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80959-1-PBS cible Ribosomal protein L4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ribosomal protein L4 Protéine recombinante Ag28728
• Nom complet: ribosomal protein L4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC009888
• Symbole du gène: Ribosomal protein L4
• Identification du gène (NCBI): 6124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

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