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Anticorps Polyclonal de lapin anti-Rootletin

Rootletin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55485-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of mouse brain tissue using Rootletin Polyclonal antibody (55485-1-AP)

    WB analysis of mouse brain using 55485-1-AP (same clone as 55485-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55485-1-AP (Rootletin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Rootletin Polyclonal antibody (55485-1-AP)

    IF Staining of hTERT-RPE1 using 55485-1-AP (same clone as 55485-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Rootletin antibody (55485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 55485-1-PBS in a different storage buffer formulation.

Informations sur le produit

55485-1-PBS cible Rootletin dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet ciliary rootlet coiled-coil, rootletin
Masse moléculaire calculée 229 kDa
Poids moléculaire observé228 kDa
Numéro d’acquisition GenBankNM_014675
Symbole du gène Rootletin
Identification du gène (NCBI) 9696
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.

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