Anticorps Monoclonal anti-S100B

S100B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G10F4

N° de cat : 66616-1-PBS

Synonymes

S100 beta, 1G10F4, Protein S100 B, Protein S100-B, S100 calcium-binding protein B

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Galerie de données de validation

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  WB analysis using 66616-1-Ig (same clone as 66616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66616-1-PBS cible S100B dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100B Protéine recombinante Ag7440
• Nom complet: S100 calcium binding protein B
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC001766
• Symbole du gène: S100 Beta
• Identification du gène (NCBI): 6285
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

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