Anticorps Polyclonal de lapin anti-S100A10

• Phare
• Validé par KD/KO

S100A10 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 11250-1-AP

Synonymes

42C, ANX2L, ANX2LG, CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

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Galerie de données de validation

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  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11250-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human skin using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11250-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of HepG2 using 11250-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11250-1-AP

  Immunofluorescent analysis of HeLa cells using 11250-1-AP (S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11250-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human S100A10 (11250-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, cellules HeLa, cellules HT-29, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cutané humain, tissu de cancer du col de l'utérus humain, tissu de cancer du pancréas humain, tissu de gliome humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

11250-1-AP cible S100A10 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A10 Protéine recombinante Ag1779
• Nom complet: S100 calcium binding protein A10
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC015973
• Symbole du gène: S100A10
• Identification du gène (NCBI): 6281
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.; Authors - Yunwei Lou
mouse	WB,IP	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu ; KD Validated
human	IF	J Nanobiotechnology; Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury; Authors - Xiao Zhai
human	WB	J Med Chem; 5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.; Authors - Mingming Wei
human,mouse	IHC	Cancers (Basel); S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis.; Authors - Alamelu G Bharadwaj ; KO Validated
mouse	WB	J Neurosci; Blocking the Thrombin Receptor Promotes Repair of Demyelinated Lesions in the Adult Brain.; Authors - Hyesook Yoon

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