Anticorps Monoclonal anti-S100A10

S100A10 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3F9

N° de cat : 66227-1-Ig

Synonymes

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

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Galerie de données de validation

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  WB analysis of human lung using 66227-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66227-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Saos-2 using 66227-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, Saos-2 cells

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66227-1-Ig cible S100A10 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100A10 Protéine recombinante Ag23596
• Nom complet: S100 calcium binding protein A10
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC015973
• Symbole du gène: S100A10
• Identification du gène (NCBI): 6281
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.