Anticorps Recombinant de lapin anti-S100A14

• Phare
• Validé par KD/KO

S100A14 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240528B6

N° de cat : 83559-5-PBS

Synonymes

240528B6, Protein S100 A14, Protein S100-A14, S100 calcium-binding protein A14, S100A15

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Galerie de données de validation

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  WB analysis of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

  WB result of S100A14 antibody (83559-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83559-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

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Informations sur le produit

83559-5-PBS cible S100A14 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: S100A14 Protéine recombinante Ag0757
• Nom complet: S100 calcium binding protein A14
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC005019
• Symbole du gène: S100A14
• Identification du gène (NCBI): 57402
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

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