Anticorps Polyclonal de lapin anti-S100A16

• Phare
• Validé par KD/KO

S100A16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 11456-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11456-1-AP (same clone as 11456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11456-1-AP (same clone as 11456-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 11456-1-AP (same clone as 11456-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 11456-1-AP (same clone as 11456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using S100A16 antibody (11456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11456-1-PBS cible S100A16 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A16 Protéine recombinante Ag2023
• Nom complet: S100 calcium binding protein A16
• Masse moléculaire calculée: 103 aa, 12 kDa
• Poids moléculaire observé: 9-12 kDa
• Numéro d’acquisition GenBank: BC019099
• Symbole du gène: S100A16
• Identification du gène (NCBI): 140576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

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