Anticorps Polyclonal de lapin anti-S100A4

• Phare
• Validé par KD/KO

S100A4 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-16105

Synonymes

18A2, 42A, Calvasculin, CAPL, FSP1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

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Galerie de données de validation

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  IF Staining of HeLa using CL594-16105

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 S100A4 antibody (CL594-16105) at dilution of 1:200.

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  FC experiment of HeLa using CL594-16105

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human S100A4 (CL594-16105) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-16105 cible S100A4 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A4 Protéine recombinante Ag9019
• Nom complet: S100 calcium binding protein A4
• Masse moléculaire calculée: 101 aa, 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC016300
• Symbole du gène: S100A4
• Identification du gène (NCBI): 6275
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C