Anticorps Monoclonal anti-S100A9

S100A9 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E12C1

N° de cat : 68900-1-Ig

Synonymes

1E12C1, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

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Galerie de données de validation

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  WB analysis of human saliva using 68900-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-468 using 68900-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1376 using 68900-1-Ig

  HT-1376 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SK-BR-3 using 68900-1-Ig

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 68900-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using S100A9 antibody (68900-1-Ig, Clone: 1E12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: salive humain, cellules MDA-MB-468
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68900-1-Ig cible S100A9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100A9 Protéine recombinante Ag27025
• Nom complet: S100 calcium binding protein A9
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC047681
• Symbole du gène: S100A9
• Identification du gène (NCBI): 6280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

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