Anticorps Recombinant de lapin anti-S100A9

S100A9 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240539G9

N° de cat : 83578-2-RR

Synonymes

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

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Galerie de données de validation

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  WB analysis of MCF-7 using 83578-2-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83578-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 83578-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 83578-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83578-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HL-60, cellules THP-1
• Résultats positifs en IF/ICC: cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83578-2-RR cible S100A9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: S100A9 Protéine recombinante Ag25764
• Nom complet: S100 calcium binding protein A9
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC047681
• Symbole du gène: S100A9
• Identification du gène (NCBI): 6280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

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