Anticorps Polyclonal de lapin anti-RPS6KB2

RPS6KB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15268-1-PBS

Synonymes

p70 S6 kinase beta, S6K2, EC:2.7.11.1, KLS, P70 beta

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Galerie de données de validation

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  WB analysis of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15268-1-AP (same clone as 15268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15268-1-AP (same clone as 15268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15268-1-PBS cible RPS6KB2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS6KB2 Protéine recombinante Ag7371
• Nom complet: ribosomal protein S6 kinase, 70kDa, polypeptide 2
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC000094
• Symbole du gène: RPS6KB2
• Identification du gène (NCBI): 6199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).

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