Anticorps Polyclonal de lapin anti-SAGE1

SAGE1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30914-1-PBS

Synonymes

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14

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Galerie de données de validation

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  IHC staining of human skin cancer using 30914-1-AP (same clone as 30914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 30914-1-AP (same clone as 30914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30914-1-AP (same clone as 30914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 30914-1-AP (same clone as 30914-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30914-1-AP (same clone as 30914-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30914-1-PBS cible SAGE1 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAGE1 Protéine recombinante Ag34216
• Nom complet: sarcoma antigen 1
• Masse moléculaire calculée: 99kd
• Numéro d’acquisition GenBank: NM_018666.2
• Symbole du gène: SAGE1
• Identification du gène (NCBI): 55511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

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