Anticorps Monoclonal anti-SALL4

SALL4 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2B5B2

N° de cat : 66236-1-Ig

Synonymes

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

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Galerie de données de validation

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  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

66236-1-Ig cible SALL4 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SALL4 Protéine recombinante Ag17480
• Nom complet: sal-like 4 (Drosophila)
• Masse moléculaire calculée: 1053 aa, 112 kDa
• Poids moléculaire observé: 165 kDa
• Numéro d’acquisition GenBank: BC111714
• Symbole du gène: SALL4
• Identification du gène (NCBI): 57167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta
-catenin and activate the Wnt/beta
-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

Publications

Species	Application	Title
human	WB	Cancer Chemother Pharmacol; Sorafenib combined with dasatinib therapy inhibits cell viability, migration, and angiogenesis synergistically in hepatocellular carcinoma.; Authors - Chiung-Chi Cheng
human	IHC	Pediatr Surg Int; SALL4 is a useful marker for pediatric yolk sac tumors.; Authors - Peng Wu