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Anticorps Recombinant de lapin anti-SALL4

SALL4 Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230284C4

N° de cat : 83039-4-PBS

Synonymes

Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4, DRRS

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SALL4 Recombinant antibody (83039-4-RR)

    IF Staining of Caco-2 using 83039-4-RR (same clone as 83039-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83039-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SALL4 Recombinant antibody (83039-4-RR)

    IF Staining of Caco-2 using 83039-4-RR (same clone as 83039-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83039-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83039-4-RR

    Affinity and Kinetic Characterization of 83039-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83039-4-RR against Human SALL4 were performed. The affinity constant is 0.18 nM.

Informations sur le produit

83039-4-PBS cible SALL4 dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène SALL4 Protéine recombinante Ag16076
Nom complet sal-like 4 (Drosophila)
Masse moléculaire calculée 1053 aa, 112 kDa
Numéro d’acquisition GenBankBC111714
Symbole du gène SALL4
Identification du gène (NCBI) 57167
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

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