Anticorps Recombinant de lapin anti-SAMD4A

SAMD4A Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251369F11

N° de cat : 86530-1-RR

Synonymes

hSmaug1, KIAA1053, Protein Smaug homolog 1, SAM domain-containing protein 4A, SAMD4

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Galerie de données de validation

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  WB analysis using 86530-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86530-1-RR (SAMD4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86530-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86530-1-RR against Human SAMD4A were performed. The affinity constant is 2.33 nM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86530-1-RR cible SAMD4A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SAMD4A Protéine recombinante Ag11273
• Nom complet: sterile alpha motif domain containing 4A
• Masse moléculaire calculée: 529 aa, 59 kDa
• Poids moléculaire observé: 70-71 kDa
• Numéro d’acquisition GenBank: BC021726
• Symbole du gène: SAMD4A
• Identification du gène (NCBI): 23034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAMD4A is also named as KIAA1053, SAMD4, SMAUG1, hSmaug1 and belongs to the SMAUG family. SAMD4A acts as a translational repressor of SRE-containing messengers. (PMID:16221671). In particular, as orthologous alternative splicing isoforms, mouse Samd4a E-form and human SAMD4A C-form containing the SAM domain were specific to testes. Samd4a/SAMD4A plays an essential role in normal spermatogenesis, and SAMD4A, as a spermatid specific marker, can be used for subcategorizing nonobstructive azoospermia patients (PMID:36274854). In mammalian cells, human SAMD4A can form a kind of mRNA‐silencing foci in the cytoplasm, acting differently from processing body and stress granules. Recently, it was reported that human SAMD4A can protect the host from viral infection by degrading viral RNA (PMID: 32341522). Indition, 14-3-3s negatively regulate the localization of the RNA-binding protein SAMD4A to cytoplasmic granules and inhibit its activity as a translational repressor (PMID: 36931259).

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