Anticorps Recombinant de lapin anti-SAMHD1

SAMHD1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251815H5

N° de cat : 86798-1-PBS

Synonymes

DCIP, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, EC:3.1.5.-

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Galerie de données de validation

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  WB analysis using 86798-1-RR (same clone as 86798-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86798-1-RR (SAMHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86798-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86798-1-RR (same clone as 86798-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (86798-1-RR, Clone: 251815H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86798-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86798-1-PBS cible SAMHD1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SAMHD1 Protéine recombinante Ag3287
• Nom complet: SAM domain and HD domain 1
• Masse moléculaire calculée: 626 aa, 72 kDa
• Poids moléculaire observé: 64-72 kDa
• Numéro d’acquisition GenBank: BC036450
• Symbole du gène: SAMHD1
• Identification du gène (NCBI): 25939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

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