Anticorps Polyclonal de lapin anti-Sam50

• Phare
• Validé par KD/KO

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 20824-1-AP

Synonymes

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

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Galerie de données de validation

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  WB analysis using 20824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20824-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20824-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20824-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20824-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20824-1-AP (Sam50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules A431, tissu ovarien de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

20824-1-AP cible Sam50 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Sam50 Protéine recombinante Ag14799
• Nom complet: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007830
• Symbole du gène: Sam50
• Identification du gène (NCBI): 25813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Publications

Species	Application	Title
human	WB	Elife; QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.; Authors - Virginia Guarani
human	WB	Cell Rep; A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.; Authors - Timothy D Martin ; KD Validated
mouse	WB	Aging Cell; 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex; Authors - Zer Vue
rat	WB	Free Radic Biol Med; Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.; Authors - Run-Qing Xue
human	WB	J Cell Biol; The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy; Authors - Mehmet Oguz Gok
pig	IF	Elife; Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system; Authors - Dalen Zuidema

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