Anticorps Polyclonal de lapin anti-SARM1

SARM1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 28625-1-AP

Synonymes

EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR

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Galerie de données de validation

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  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 28625-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of SH-SY5Y using 28625-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody (28625-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HEK-293, cellules Neuro-2a, cellules Raji, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

28625-1-AP cible SARM1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARM1 Protéine recombinante Ag29531
• Nom complet: sterile alpha and TIR motif containing 1
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_015077
• Symbole du gène: SARM1
• Identification du gène (NCBI): 23098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

Publications

Species	Application	Title
mouse	IF	Neurosci Res; SARM1 is essential for NMDA receptor-dependent endocytosis of AMPA receptors in hippocampal neurons; Authors - Misaki Morishita

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anam (Verified Customer) (09-03-2025)

For IF and ICC it works really well

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Cell culture
• Primary Antibody Dilution: 1:200 or 1:1000
• Cell Tissue Type: Schwann cells, Stem cells

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