Anticorps Polyclonal de lapin anti-SARM1

SARM1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28625-1-PBS

Synonymes

EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR

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Galerie de données de validation

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  WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

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  WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody (28625-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28625-1-PBS cible SARM1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARM1 Protéine recombinante Ag29531
• Nom complet: sterile alpha and TIR motif containing 1
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_015077
• Symbole du gène: SARM1
• Identification du gène (NCBI): 23098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

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