Anticorps Monoclonal anti-SARS-CoV-2 Nucleocapsid Phosphoprotein

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Virus

Applications

WB, ELISA, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B3C3

N° de cat : 67666-1-PBS

Synonymes

nucleocapsid phosphoprotein, 1B3C3, sars cov 2

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Galerie de données de validation

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  WB analysis using 67666-1-Ig (same clone as 67666-1-PBS)

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50061-1

  Sandwich ELISA standard curve of MP50061-1, Virus 2019-nCOV nucleocapsid phosphoprotein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67666-1-PBS. Detection antibody: HRP-conjugated 67666-2-PBS. Standard: Ag30676. Range: 0.5-20 ng/mL.

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  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67666-1-PBS cible SARS-CoV-2 Nucleocapsid Phosphoprotein dans les applications de WB, ELISA, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 Nucleocapsid Phosphoprotein Protéine recombinante Ag30676
• Nom complet: COVID-19 N Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 N Protein
• Identification du gène (NCBI): 43740575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

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