Anticorps Polyclonal de lapin anti-SART3

SART3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 18025-1-AP

Synonymes

DSAP1, hSART 3, KIAA0156, P100, p110

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Galerie de données de validation

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18025-1-AP

  IP result of anti-SART3 (IP:18025-1-AP, 4ug; Detection:18025-1-AP 1:700) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu cérébral humain, tissu cutané humain, tissu de cancer du poumon humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

18025-1-AP cible SART3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SART3 Protéine recombinante Ag12590
• Nom complet: squamous cell carcinoma antigen recognized by T cells 3
• Masse moléculaire calculée: 963 aa, 110 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC093784
• Symbole du gène: SART3
• Identification du gène (NCBI): 9733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

Publications

Species	Application	Title
human	WB	Sci Adv; Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection; Authors - Yiyao Huang
mouse,human	IP	RNA; 7SL RNA in Vertebrate Red Blood Cells.; Authors - Gaëlle J S Talhouarne
human	WB,IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
		Mol Cell Biol; USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.; Authors - Jaehyun Kim
human	IHC	Aging (Albany NY); SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma; Authors - Jusen Nong

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