Anticorps Polyclonal de lapin anti-SASH1

SASH1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27689-1-PBS

Synonymes

KIAA0790, PEPE1, Proline-glutamate repeat-containing protein, RP3 323M4.1, SAM and SH3 domain-containing protein 1

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Galerie de données de validation

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  WB analysis using 27689-1-AP (same clone as 27689-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27689-1-AP (SASH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27689-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 27689-1-AP (same clone as 27689-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SASH1 antibody (27689-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 27689-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27689-1-PBS cible SASH1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SASH1 Protéine recombinante Ag26771
• Nom complet: SAM and SH3 domain containing 1
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_015278
• Symbole du gène: SASH1
• Identification du gène (NCBI): 23328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SAM and SH3 domain-containing protein 1 (SASH1, also known as KIAA0790 and PEPE1) is a multidomain protein implicated in various cellular processes, including cell adhesion, migration, and apoptosis. It has been identified as a novel Eph receptor-binding partner through SAM-SAM domain interactions, which are critical for the recruitment and binding of downstream molecules in the Eph signaling pathway (PMID: 37619706). SASH1 is also known to regulate signaling through focal adhesion kinase (FAK) and AKT/PI3K and promote apoptosis. Mutations in SASH1 have been associated with cancers, and it is suggested as a potential therapeutic target in non-small cell lung cancer (PMID: 33122723).

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