Anticorps Polyclonal de lapin anti-SBDS

SBDS Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17618-1-AP

Synonymes

CGI 97, SBDS, SDS, SWDS

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Galerie de données de validation

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  WB analysis using 17618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17618-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17618-1-AP (SBDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17618-1-AP

  IP Result of anti-SBDS (IP:17618-1-AP, 4ug; Detection:17618-1-AP 1:300) with HL-60 cells lysate 3040ug.

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  IHC staining of human pancreas cancer using 17618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17618-1-AP (SBDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17618-1-AP (SBDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293T, cellules HL-60, cellules SH-SY5Y
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17618-1-AP cible SBDS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SBDS Protéine recombinante Ag11814
• Nom complet: Shwachman-Bodian-Diamond syndrome
• Masse moléculaire calculée: 250 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC065700
• Symbole du gène: SBDS
• Identification du gène (NCBI): 51119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. This antibody is a rabbit polyclonal antibody raised against a full-length human SBDS protein, recognizes specifically the 29kd SBDS protein.

Publications

Species	Application	Title
human	WB	Oncogenesis; The HIF/PHF8/AR axis promotes prostate cancer progression.; Authors - D Tong
rat	WB	Brain Res Bull; Cerebral ischemia-reperfusion causes a down regulation of HCN1 expression via enhancing the nuclear NRSF-HDAC4 gathering that contributes to neuron damage.; Authors - Pan Luo
human	IHC,IF	Am J Transl Res; Three RNA-binding protein signature as a novel biomarker to predict endometrial cancer prognosis; Authors - Bo Wang