Anticorps Monoclonal anti-SBDS

• Phare
• Validé par KD/KO

SBDS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1F8A10

N° de cat : 67200-1-Ig

Synonymes

1F8A10, CGI 97, CGI-97, SDS, Shwachman-Bodian-Diamond syndrome protein

---

Galerie de données de validation

• 

  WB analysis using 67200-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67200-1-Ig (SBDS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67200-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67200-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67200-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of HepG2 using 67200-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

67200-1-Ig cible SBDS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SBDS Protéine recombinante Ag11731
• Nom complet: Shwachman-Bodian-Diamond syndrome
• Masse moléculaire calculée: 250 aa, 29 kDa
• Poids moléculaire observé: 29-31 kDa
• Numéro d’acquisition GenBank: BC065700
• Symbole du gène: SBDS
• Identification du gène (NCBI): 51119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.; Authors - David P Dannheisig ; KD Validated
Human		Cancer Med; Development and validation of a leukocyte-associated immunoglobulin-like receptor-1 prognostic signature for lower-grade gliomas.; Authors - Zhansheng Fang

{{ptg:RelatedPrimaryAntibodies}}