Anticorps Polyclonal de lapin anti-SCAMP1

SCAMP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 15327-1-AP

Synonymes

SCAMP, SCAMP1, SCAMP37

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Galerie de données de validation

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  WB analysis of mouse brain using 15327-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15327-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15327-1-AP

  IP result of anti-SCAMP1 (IP:15327-1-AP, 4ug; Detection:15327-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of mouse brain using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu pancréatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15327-1-AP cible SCAMP1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAMP1 Protéine recombinante Ag3258
• Nom complet: secretory carrier membrane protein 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC034048
• Symbole du gène: SCAMP1
• Identification du gène (NCBI): 9522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IP	Int J Mol Sci; Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication.; Authors - Tanzeel Yousaf
human	IHC	Cell Death Dis; MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer.; Authors - Jayakumar Vadakekolathu
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao
human	WB,IHC	J Cancer; SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer; Authors - Gang Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olga (Verified Customer) (01-17-2025)

Works perfectly in Western. Gives a clear single band of the corresponding size in almost all tissues and cell lines tested. Recognizes mouse and human Scamp1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tissues (heart, lung, liver, forebrain), mouse primary cerebellar neurons, Neuro2A, HEK293T

SCAMP1 Antibody Western Blot validation (1:1000 dilution) in Mouse tissues (heart, lung, liver, forebrain), mouse primary cerebellar neurons, Neuro2A, HEK293T (Cat no:15327-1-AP)

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