Anticorps Polyclonal de lapin anti-SCAMP3

• Phare
• Validé par KD/KO

SCAMP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 26888-1-AP

Synonymes

C1orf3, PROPIN1, Secretory carrier-associated membrane protein 3

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Galerie de données de validation

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  WB analysis using 26888-1-AP

  Huh7 cell lysates were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26888-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26888-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26888-1-AP

  IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with HepG2 cells lysate 1480 ug.

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  IP experiment of mouse heart using 26888-1-AP

  IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with mouse heart tissue lysate 1880 ug.

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  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules A549, cellules K-562
• Résultats positifs en IP: cellules HepG2, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

26888-1-AP cible SCAMP3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAMP3 Protéine recombinante Ag25256
• Nom complet: secretory carrier membrane protein 3
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000161
• Symbole du gène: SCAMP3
• Identification du gène (NCBI): 10067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Mol Cell; mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux.; Authors - Florian Beaumatin ; KD Validated
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao
human	WB	Sci Rep; MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis.; Authors - Agné Kulyté
human	WB,IHC	Onco Targets Ther; SCAMP3 Promotes Glioma Proliferation and Indicates Unfavorable Prognosis via Multiple Pathways.; Authors - Chunliu Li ; KD Validated
human	WB	Onco Targets Ther; Bioinformatics Analysis and RNA-Sequencing of SCAMP3 Expression and Correlated Gene Regulation in Hepatocellular Carcinoma.; Authors - Shan-Shan Han
human	IF	Int J Biol Macromol; Fungal antitumor protein D1 is internalized via endocytosis and inhibits non-small cell lung cancer proliferation through MAPK signaling pathway; Authors - Min-Hui Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

This antibody provided some convincing IF staining but detected many unspecific bands when western blotting HUVEC lysate.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 200 IF 1 in 1000 WB
• Cell Tissue Type: HUVEC

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