Anticorps Polyclonal de lapin anti-SCAP

SCAP Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 12266-1-AP

Synonymes

KIAA0199, SCAP, SREBF chaperone, SREBP cleavage-activating protein, Sterol regulatory element-binding protein cleavage-activating protein

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Galerie de données de validation

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  WB analysis of HEK-293 using 12266-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12266-1-AP (SCAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12266-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12266-1-AP (SCAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 12266-1-AP

  Immunofluorescent analysis of HEK-293 cells using 12266-1-AP (SCAP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

12266-1-AP cible SCAP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAP Protéine recombinante Ag2937
• Nom complet: SREBF chaperone
• Masse moléculaire calculée: 1279 aa, 140 kDa
• Poids moléculaire observé: 140 kDa, 96 kDa
• Numéro d’acquisition GenBank: BC020987
• Symbole du gène: SCAP
• Identification du gène (NCBI): 22937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; PMID: 24843127; Authors - Chong Wee Liew
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	WB	bioRxiv; Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation; Authors - Ting Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (08-14-2020)

Nice staining we got in our tissue sections

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human colon cancer tissue