Anticorps Polyclonal de lapin anti-SCAP

SCAP Polyclonal Antibody for IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (2)

N° de cat : 12266-1-AP

Synonymes

SREBP cleavage-activating protein, SREBP cleavage activating protein, SREBFchaperone, SREBF chaperone, PSEC0227

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Galerie de données de validation

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  IP experiment of HEK-293 using 12266-1-AP

  IP result of anti-SCAP (IP:12266-1-AP, 4ug; Detection:12266-1-AP 1:600) with HEK-293 cells lysate 1610 ug.

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  IHC staining of mouse kidney using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

12266-1-AP cible SCAP dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAP Protéine recombinante Ag2937
• Nom complet: SREBF chaperone
• Masse moléculaire calculée: 1279 aa, 140 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC020987
• Symbole du gène: SCAP
• Identification du gène (NCBI): 22937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; PMID: 24843127; Authors - Chong Wee Liew
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
Human	WB	Sci Rep; TDP-43 regulates cholesterol biosynthesis by inhibiting sterol regulatory element-binding protein 2.; Authors - Naohiro Egawa
human	WB	bioRxiv; Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation; Authors - Ting Wang
mouse	WB	Biochem Biophys Rep; Hepatic HSD17B6 is dispensable for diet-induced fatty liver disease in mice; Authors - Delong Yuan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (09-18-2025)

Very good quality SCAP antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for IB
• Cell Tissue Type: CRC cells

FH

Iram (Verified Customer) (08-14-2020)

Nice staining we got in our tissue sections

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human colon cancer tissue

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