Anticorps Polyclonal de lapin anti-SCAP

SCAP Polyclonal Antibody for IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12266-1-PBS

Synonymes

SREBP cleavage-activating protein, SREBP cleavage activating protein, SREBFchaperone, SREBF chaperone, PSEC0227

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Galerie de données de validation

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  IP experiment of HEK-293 using 12266-1-AP (same clone as 12266-1-PBS)

  IP result of anti-SCAP (IP:12266-1-AP, 4ug; Detection:12266-1-AP 1:600) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12266-1-AP (same clone as 12266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12266-1-AP (same clone as 12266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12266-1-PBS cible SCAP dans les applications de IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCAP Protéine recombinante Ag2937
• Nom complet: SREBF chaperone
• Masse moléculaire calculée: 1279 aa, 140 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC020987
• Symbole du gène: SCAP
• Identification du gène (NCBI): 22937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

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