Anticorps Polyclonal de lapin anti-SCD1

• Phare
• Validé par KD/KO

SCD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28678-1-PBS

Synonymes

SCD, EC:1.14.19.1, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase

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Galerie de données de validation

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  WB analysis using 28678-1-AP (same clone as 28678-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28678-1-AP (SCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)

  WB result of SCD antibody (28678-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCD transfected A431 cells. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 28678-1-AP (same clone as 28678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (28678-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28678-1-PBS cible SCD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCD1 Protéine recombinante Ag29156
• Nom complet: stearoyl-CoA desaturase (delta-9-desaturase)
• Masse moléculaire calculée: 355 aa, 41 kDa
• Poids moléculaire observé: 28-42 kDa
• Numéro d’acquisition GenBank: BC005807
• Symbole du gène: SCD
• Identification du gène (NCBI): 6319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

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