Anticorps Recombinant de lapin anti-SCF

SCF Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240787H1

N° de cat : 83707-2-PBS

Synonymes

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

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Galerie de données de validation

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  WB analysis using 83707-2-RR (same clone as 83707-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83707-2-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 83707-2-RR (same clone as 83707-2-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83707-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00664-1

  Sandwich ELISA standard curve of MP00664-1, Rat SCF Recombinant Matched Antibody Pair - PBS only. 83707-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1092. 83707-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL

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  Sample test of MP00664-1

  Serum of eight rats was measured. The rat SCF concentration of detected samples was determined to be 191.0 pg/mL with a range of 14.0 - 481.6 pg/mL.

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  Cytometric bead array standard curve of MP00664-1

  Cytometric bead array standard curve of MP00664-1, RAT SCF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83707-1-PBS. Detection antibody: 83707-2-PBS. Standard: Eg1092. Range: 0.313-20 ng/mL

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  Affinity and Kinetic Characterization of 83707-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

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Informations sur le produit

83707-2-PBS cible SCF dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: KIT ligand
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 28 kDa, 45 kDa
• Numéro d’acquisition GenBank: NM_021843.4
• Symbole du gène: Kitlg
• Identification du gène (NCBI): 60427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.

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