Anticorps Recombinant de lapin anti-SCF

SCF Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240787H1

N° de cat : 83707-2-RR

Synonymes

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

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Galerie de données de validation

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  WB analysis using 83707-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 83707-2-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83707-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules Neuro-2a, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83707-2-RR cible SCF dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: KIT ligand
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 28 kDa, 45 kDa
• Numéro d’acquisition GenBank: NM_021843.4
• Symbole du gène: Kitlg
• Identification du gène (NCBI): 60427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.

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