Anticorps Polyclonal de lapin anti-Secretogranin II

Secretogranin II Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 20357-1-AP

Synonymes

CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 20357-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20357-1-AP (Secretogranin II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 20357-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 20357-1-AP (Secretogranin II antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu pancréatique de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20357-1-AP cible Secretogranin II dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Secretogranin II Protéine recombinante Ag14215
• Nom complet: secretogranin II (chromogranin C)
• Masse moléculaire calculée: 617 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC022509
• Symbole du gène: SCG2
• Identification du gène (NCBI): 7857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.

Publications

Species	Application	Title
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
mouse,rat	WB,IF	J Cell Biol; SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.; Authors - Hongfei Xu
human	WB,IHC,CoIP	Mol Oncol; Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.; Authors - Chao Fang
human	IHC	Front Mol Biosci; Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.; Authors - Hufei Wang
human	IHC	Front Physiol; Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis; Authors - Xiaoshuo Lv
mouse	WB	Front Neurol; Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice; Authors - Jingyu Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Wojciech (Verified Customer) (03-02-2023)

The antibody worked very well. The dilution used was 1:1000 in 5% non-fat milk. The single detected band size was around 75 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines