Anticorps Polyclonal de lapin anti-Secretogranin III

• Phare
• Validé par KD/KO

Secretogranin III Polyclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 10954-1-AP

Synonymes

SCG3, UNQ2502/PRO5990, SgIII, Secretogranin-3, Secretogranin 3

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Galerie de données de validation

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  IHC staining of mouse pancreas using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 10954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Secretogranin III antibody (10954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 10954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Secretogranin III antibody (10954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu pancréatique de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Informations sur le produit

10954-1-AP cible Secretogranin III dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Secretogranin III Protéine recombinante Ag1397
• Nom complet: secretogranin III
• Masse moléculaire calculée: 53 kDa
• Numéro d’acquisition GenBank: BC014539
• Symbole du gène: Secretogranin 3
• Identification du gène (NCBI): 29106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

Publications

Species	Application	Title
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB,IHC	Cancer Lett; The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.; Authors - Yue Wang ; KD Validated
mouse	WB,IHC	Cereb Cortex; Secretogranin III is an astrocyte granin that is overexpressed in reactive glia.; Authors - Paco Sonia S
human, mouse	IHC	Brain Pathol; Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.; Authors - Virginia Plá
Mouse	WB	Epigenetics Chromatin; Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck
mouse	IHC,IF	J Endocrinol; Secretogranin II binds to secretogranin III and forms secretory granules with orexin, neuropeptide Y, and POMC.; Authors - Hotta Kikuko K

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