Anticorps Monoclonal anti-SCGF

SCGF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4H11E4

N° de cat : 60295-1-PBS

Synonymes

CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A

---

Galerie de données de validation

• 

  WB analysis using 60295-1-Ig (same clone as 60295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60295-1-Ig (SCGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 60295-1-Ig (same clone as 60295-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60295-1-Ig (CLEC11A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

60295-1-PBS cible SCGF dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SCGF Protéine recombinante Ag21365
• Nom complet: C-type lectin domain family 11, member A
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC005810
• Symbole du gène: SCGF
• Identification du gène (NCBI): 6320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.

{{ptg:RelatedPrimaryAntibodies}}