Anticorps Polyclonal de lapin anti-SCGN

SCGN Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

N° de cat : 14919-1-AP

Synonymes

CALBL, SCGN, SECRET, Secretagogin, SEGN, setagin

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Galerie de données de validation

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  WB analysis of mouse pancreas using 14919-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 14919-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14919-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14919-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 14919-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14919-1-AP

  IP Result of anti-SCGN (IP:14919-1-AP, 4ug; Detection:14919-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu cérébral de rat, tissu cérébral humain fœtal, tissu de côlon de souris, tissu d'estomac de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14919-1-AP cible SCGN dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCGN Protéine recombinante Ag6736
• Nom complet: secretagogin, EF-hand calcium binding protein
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000336
• Symbole du gène: SCGN
• Identification du gène (NCBI): 10590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.